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Message: ERA-EDTA 2019 Symposium: Vascular calcification in kidney disease: Epigenetics as a novel approach?

Holy alkaline phosphatase Batman! Huge effects on alkaline phosphatase.

This is the abstract for the oral presentation tomorrow by Dr. Kulikowski. Both the poster presentation by Dr. Kalantar-Zadeh on FGF23 (see abstract here) and Dr. Kulikowski's oral presentation on alkaline phosphatase happen tomorrow. 

FO080 - APABETALONE, AN INHIBITOR OF BET PROTEINS, IMPROVES CARDIOVASCULAR RISK AND REDUCES ALKALINE PHOSPHATASE IN BOTH CVD PATIENTS AND PRIMARY HUMAN CELL CULTURE SYSTEMSDean GilhamLaura TsujikawaSylwia WasiakChristopher HallidayKen LebiodaKamyar Kalantar-ZadehSrinivasanBeddhuVincent BrandenburgMarcello TonelliRavi JahagirdarNorman C. W. WongCarmine ZoccaliJan O.JohanssonMichael SweeneyMathias HaarhausEwelina Kulikowski
Introduction and Aims

Apabetalone is an orally active inhibitor of bromodomain and extraterminal (BET) proteins - epigenetic readers modulating gene expression by bridging acetylated histones or transcription factors with transcriptional machinery. In phase 2 clinical trials, apabetalone reduced major adverse cardiac events (MACE) in patients with cardiovascular disease (CVD), resulting in 44% relative risk reduction on top of standard of care. Chronic kidney disease (CKD) is frequently accompanied by cardiovascular complications, which remain resistant to current therapies. Elevated serum alkaline phosphatase (ALP) contributes to vascular calcification (VC) and endothelial dysfunction. Accordingly, serum ALP is emerging as an independent and novel predictor of MACE and of all-cause mortality.

Methods

Serum ALP levels were examined post-hoc in CVD patients receiving apabetalone in the 3 month (ASSERT) and 6 month (SUSTAIN & ASSURE) phase 2 trials, including a subset with impaired renal function (eGFR<60 mL/min/1.73m2). Effects of BET inhibitors (BETi) on expression of tissue-nonspecific ALP (TNAP; gene symbol ALPL) was determined in cultured primary human hepatocytes (PHH), the HepaRG cell line, primary human vascular smooth muscle cells (VSMCs) during trans-differentiation to calcifying cells, and vascular endothelial cells. ALP enzyme activity was measured in enzymatic assays.

Results

In phase 2 trials, baseline serum ALP independently predicted MACE (hazard ratio [HR] per SD 1.6, 95% CI 1.2-2.2, p=0.001). In ASSERT, apabetalone dose dependently reduced serum ALP (n=74-76/group; p<0.001 median change vs. placebo). Patients in phase 2 on apabetalone (n=553) had greater reductions in ALP than placebo (n=242; p<0.001). ALP reduction by apabetalone was associated with reduction in MACE (HR 0.58 per SD, 95% CI 0.44-0.77, p<0.001). In the subgroup with eGFR<60, patients on apabetalone (n=69) also had lower on-treatment serum ALP (p=0.008) vs. placebo (n=22). Liver-derived TNAP accounts for ≈50% of ALP enzyme activity in serum. In PHH & HepaRG cells, apabetalone suppressed ALPL expression by 60-80%. Vascular expression of ALPL also contributes to VC & cardiovascular risk. Compared to basal conditions, trans-differentiation of VSMCs to calcifying cells resulted in 2.5x increase in ALPL expression. Apabetalone or JQ1 (BETi with different chemical scaffolds) countered extracellular calcium deposition and suppressed ALPL gene expression, TNAP protein levels, and enzyme activity. Apabetalone downregulated ALPL expression in human aortic endothelial cells, umbilical vein endothelial cells, and brain microvascular endothelial cells by 50-70%.

Conclusions

Apabetalone dose dependently lowers serum ALP in patients, which is associated with reduction in cardiovascular events. Decreased ALPL expression in cultured hepatocytes with apabetalone is consistent with reduced serum ALP in patients. In addition, apabetalone downregulates ALPL expression in vascular cell types including VSMCs and endothelial cells, while reducing VSMC calcification. Involvement of BET proteins in VSMC calcification is a novel discovery. Our data indicate apabetalone has potential to decrease progression of pathological VC and contribute to positive cardiovascular outcomes in CVD patients. The impact of apabetalone treatment on biomarkers, renal function, and CVD outcomes is being evaluated in the phase 3 BETonMACE trial (NCT02586155).

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